Composite

Part:BBa_I716251:Design

Designed by: Vaibhavi Umesh   Group: iGEM07_Berkeley_UC   (2007-06-05)


Composite part consisting of Ptet + RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 785
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 785
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 770
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 785
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 785
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1872


Design Notes

Biobrickificreation of composite Ptet + RFP (product = I716251)

Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) Product is I716251

No Oligos Needed
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Source

The source of these parts are:

pBca9145-Bca1145

pBca9145-Bca9205

References